CNPase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85999

Novus Biologicals, part of Bio-Techne
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NBP1-85999
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CNPase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFG

Marker

Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNPase Antibody

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85999] - Staining in human cerebral cortex and pancreas tissues using NBP1-85999 antibody. Corresponding CNP RNA-seq data are presented for the same tissues.
Western Blot: CNPase Antibody [NBP1-85999]

Western Blot: CNPase Antibody [NBP1-85999]

Western Blot: CNPase Antibody [NBP1-85999] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP1-85999]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP1-85999]

Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP1-85999] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is show in green.
View 4 more images

Applications for CNPase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CNPase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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