Coagulation Factor III/Tissue Factor Antibody (CL3807)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61641

Novus Biologicals, part of Bio-Techne
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NBP2-61641
NBP2-61641-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3807

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Coagulation Factor III/Tissue Factor Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGEN

Epitope

SPEFTPYLET

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Coagulation Factor III/Tissue Factor Antibody (CL3807)

Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-F3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line A-431
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
View 6 more images

Applications for Coagulation Factor III/Tissue Factor Antibody (CL3807)

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor III/Tissue Factor

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

F3

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Coagulation Factor III/Tissue Factor Antibody (CL3807)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Coagulation Factor III/Tissue Factor Antibody (CL3807)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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