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Coagulation Factor III/Tissue Factor Antibody (TF9-10H10)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65221

Clone TF9-10H10 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65221
NB100-65221-0.025mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TF9-10H10

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Denatured Tissue factor isolated from human brain by the Factor VII affinity method

Reactivity Notes

Predicted cross-reactivities: Primate

Localization

Membrane; single pass type I membrane protein.

Specificity

CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation. NB100-65221 is specific for an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1-10 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor III/Tissue Factor

A series of complicated reactions take place in the body whenever a blood clot begins to form. The major initiator of these reactions is an enzyme system called the tissue factor/factor VIIa (TF/FVIIa) complex. Animal tests show that various inhibitors of the TF/FVIIa complex can minimize blood clot formation as well as inflammatory responses.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human)

Gene Symbol

F3

UniProt

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Coagulation Factor III/Tissue Factor Antibody (TF9-10H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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