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Coagulation Factor X Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33320

Novus Biologicals, part of Bio-Techne
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NBP1-33320

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rhesus Macaque (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coagulation Factor X. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (81%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coagulation Factor X Antibody

Western Blot: Coagulation Factor X Antibody [NBP1-33320]

Western Blot: Coagulation Factor X Antibody [NBP1-33320]

Western Blot: Coagulation Factor X Antibody [NBP1-33320] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320]

Immunocytochemistry/ Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320]

Immunocytochemistry/Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320] - Methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320]

Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320]

Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320] - NCI-N87 xenograft, using Factor X antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Coagulation Factor X Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coagulation Factor X

The Factor X gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoesmultiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of thetripeptide RKR. Two chai

Alternate Names

Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor

Gene Symbol

F10

Additional Coagulation Factor X Products

Product Documents for Coagulation Factor X Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Coagulation Factor X Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.