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COASY Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15934

Novus Biologicals, part of Bio-Techne
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NBP2-15934

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Porcine (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.

Reactivity Notes

Mouse (87%), Rat (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COASY Antibody

Western Blot: COASY Antibody [NBP2-15934]

Western Blot: COASY Antibody [NBP2-15934]

Western Blot: COASY Antibody [NBP2-15934] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: COASY Antibody [NBP2-15934]

Immunocytochemistry/ Immunofluorescence: COASY Antibody [NBP2-15934]

Immunocytochemistry/Immunofluorescence: COASY Antibody [NBP2-15934] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: COASY Antibody [NBP2-15934]

Immunohistochemistry-Paraffin: COASY Antibody [NBP2-15934]

Immunohistochemistry-Paraffin: COASY Antibody [NBP2-15934] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:500 dilution.

Applications for COASY Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, pOV-2, PPAT

Gene Symbol

COASY

UniProt

Additional COASY Products

Product Documents for COASY Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COASY Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.