Collagen II Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46876
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Sheep
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Collagen II Antibody [FITC]
Immunogen
Collagen II from human knee cartilage and bovine nasal cartilage. (Uniprot: P02458)
Reactivity Notes
This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens.
Canine reactivity reported in scientific literature (PMID: 22972852).
Avian reactivity reported in scientific literature (PMID: 24739280).
Bovine reactivity reported in scientific literature (PMID: 23688110).
Silk worm reactivity reported in scientific literature (PMID: 23845228).
Canine reactivity reported in scientific literature (PMID: 22972852).
Avian reactivity reported in scientific literature (PMID: 24739280).
Bovine reactivity reported in scientific literature (PMID: 23688110).
Silk worm reactivity reported in scientific literature (PMID: 23845228).
Specificity
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Scientific Data Images for Collagen II Antibody [FITC]
Immunohistochemistry: Collagen II Antibody [Fluorescein] [NBP2-46876] - Analysis of Tissue: human bronchiolar cartilage (shown). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Fixation: formalin fixed paraffin embedded. Antigen retrieval: 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes. Primary antibody: collagen II antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen II is extracellular. Staining: Collagen II as precipitated red signal with hematoxylin purple nuclear counterstain.
Applications for Collagen II Antibody [FITC]
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:200 - 1:1000
Immunoprecipitation
1:100
Western Blot
1:5000-1:10000
Application Notes
This product is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 50 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Collagen II
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
UniProt
Additional Collagen II Products
Product Documents for Collagen II Antibody [FITC]
Product Specific Notices for Collagen II Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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