Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Kangaroo, Rabbit

Cited:

Human, Mouse, Rat, Canine, Rabbit

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E7-D7/Col3

Format

BSA Free

Concentration

1.0 mg/ml

View all Collagen III alpha 1/COL3A1 Antibodies »

Product Specifications

Immunogen

This Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) was developed against acid-digested pepsin soluble human type III collagen

Epitope

Epitope is located approximately 100 nm from the N-terminal of the molecule

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 26373929). Mouse reactivity reported in scientific literature (PMID: 30594070).

Specificity

Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3)BSA Free [NBP1-05119]

Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3)BSA Free [NBP1-05119]

Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Western blot analysis on total proteins extracted from cells cultured with L-PRP or P-PRP. Collagen I protein band was robust in the P-PRP-treated group; collagen III alpha 1 protein band was more abundant in the L-PRP-treated cells. Image collected and cropped by CiteAb from the following publication (https://stemcellres.com/content/6/1/173) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119]

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119]

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Human bronchus tissue

Applications for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

Application
Recommended Usage

ELISA

1:800

Immunocytochemistry/ Immunofluorescence

1:100 - 1:300

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30405320). ELISA: NBP1-05119 can be used for detection of collagens by ELISA. WB: In immunoblotting NBP1-05119 detects type III collagen only in its native triple helix form. IHC: NBP1-05119 has been used successfully for immunohistology on frozen unfixed sections of human (5) and kangaroo (6) skin, and of new dog tissue associated with biomaterial implants. (7, 8) If fixation of tissue is required, acetone or ethanol is recommended. (5, 6, 7, 8).

Reviewed Applications

Read 1 review rated 5 using NBP1-05119 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM Phosphate buffer (pH 7.4), 0.5 M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Collagen III alpha 1/COL3A1

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

5. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

6. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.

7. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

8. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119

Alternate Names

COL3A1, EDS4A

Gene Symbol

COL3A1

Additional Collagen III alpha 1/COL3A1 Products

Product Documents for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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