Collagen III alpha 1/COL3A1 Antibody (C4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43316
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C4
Concentration
1 mg/ml
Product Specifications
Immunogen
Immunized with recombinant Mouse Collagen III alpha 1/COL3A1 (Uniprot#: P08121; Gln24~Pro154)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Collagen III alpha 1/COL3A1 Antibody (C4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5-20 ug/mL
Immunohistochemistry
5-20 ug/mL
Western Blot
0.01-2ug/mL
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH7.4, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen III alpha 1/COL3A1
Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).
Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).
References
1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003
2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978
3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017
4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057
Alternate Names
COL3A1, EDS4A
Gene Symbol
COL3A1
Additional Collagen III alpha 1/COL3A1 Products
Product Documents for Collagen III alpha 1/COL3A1 Antibody (C4)
Product Specific Notices for Collagen III alpha 1/COL3A1 Antibody (C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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