Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25419
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1906
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Collagen III alpha 1/COL3A1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] -
Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Collagen III antibody [HL1906] detects Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Collagen III stained by Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] -
Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] -
Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen III alpha 1/COL3A1
Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).
Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).
References
1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003
2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978
3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017
4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057
Alternate Names
COL3A1, EDS4A
Gene Symbol
COL3A1
Additional Collagen III alpha 1/COL3A1 Products
Product Documents for Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free
Product Specific Notices for Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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