Collagen III alpha 1/COL3A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15946

Novus Biologicals, part of Bio-Techne
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NBP2-15946
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Mouse, Porcine

Predicted:

Bovine (94%), Mouse (92%), Rat (92%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Collagen III alpha 1/COL3A1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III alpha 1/COL3A1. The exact sequence is proprietary.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28794502). Chicken (83%), Xenopus laevis (80%).

Localization

Secreted; extracellular space; extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen III alpha 1/COL3A1 Antibody

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946]

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946]

Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Collagen III alpha 1/COL3A1 Antibody

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] -

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Various extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha 1/COL3A1 antibody [C2C3], C-term (NBP2-15946) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Collagen III alpha 1/COL3A1 Antibody

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] -

Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Collagen III alpha 1/COL3A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID: 32345960)

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Collagen III alpha 1/COL3A1

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Alternate Names

COL3A1, EDS4A

Gene Symbol

COL3A1

Additional Collagen III alpha 1/COL3A1 Products

Product Documents for Collagen III alpha 1/COL3A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen III alpha 1/COL3A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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