Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Feline

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all Collagen IV Antibodies »

Product Specifications

Immunogen

Collagen IV from human and bovine placenta (Uniprot: P02462)

Reactivity Notes

This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Rat reactivity reported in scientific literature (PMID: 16774905)

Feline reactivity reported in scientific literature (PMID: 33091431).

Localization

Secreted and Cell surface (Potential).

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Scientific Data Images for Collagen IV Antibody [Biotin]

Immunohistochemistry-Paraffin: Collagen IV Antibody [Biotin] [NB120-6581] - Immunohistochemical staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [Biotin] [NB120-6581] - Collagen IV antibody 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.

Applications for Collagen IV Antibody [Biotin]

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1:100

Western Blot

1:2000-1:10000
Application Notes
This product is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Reviewed Applications

Read 1 review rated 5 using NB120-6581 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Collagen IV

These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV Products

Product Documents for Collagen IV Antibody [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Collagen IV Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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