Collagen IV Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26549

Novus Biologicals, part of Bio-Techne
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NBP1-26549
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Biotin
NBP1-26550
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.4 mg/ml

View all Collagen IV Antibodies »

Product Specifications

Immunogen

Goats were hyperimmunized with human type IV collagen.

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 27417518), Mouse reactivity reported in scientific literature (PMID: 32479919), Rat reactivity reported in scientific literature (PMID: 30362896).

Specificity

Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Collagen IV Antibody

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549] - Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Mouse brain tissue (12um thickness) was incubated overnight with NBP1-26549 (1:400) in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody (Alex Fluor 568-conjugated), 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type IV Collagen-UNLB; (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.
Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Col-IV in the rhesus macaque and baboon intramural tube. Images of open fallopian tube (m, o) were obtained from control animals. Occluded tube (n, p) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification 200x. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27417518) licensed under a CC-BY license.
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Applications for Collagen IV Antibody

Application
Recommended Usage

ELISA

1:1000-1:4000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
IHC, IHC-FR reported in (PMID: 32029859), ICC/IF reported in (PMID: 32479919), IHC-P reported in (PMID: 27417518).

Reviewed Applications

Read 1 review rated 5 using NBP1-26549 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Preservative

No Preservative

Concentration

0.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Collagen IV

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

Additional Collagen IV Products

Product Documents for Collagen IV Antibody

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Product Specific Notices for Collagen IV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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