Collagen V Antibody (1E2-E4/Col5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05118
![Western Blot: Collagen V Antibody (1E2-E4/Col5)BSA Free [NBP1-05118]](https://resources.bio-techne.com/images/products/Collagen-V-Antibody-1E2-E4-Col5-Western-Blot-NBP1-05118-img0001.jpg "Western Blot: Collagen V Antibody (1E2-E4/Col5)BSA Free [NBP1-05118]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Bovine, Canine, Chicken (Negative), Guinea Pig (Negative), Kangaroo, Mouse (Negative), Rabbit, Rat (Negative), Sheep
Cited:
Porcine
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E2-E4/Col5
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Collagen V Antibody (1E2-E4/Col5) - BSA Free
Immunogen
Acid-digested pepsin soluble dog type V collagen
Specificity
Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I,
III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other
connective tissue proteins (laminin, fibronectin, elastin).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Collagen V Antibody (1E2-E4/Col5) - BSA Free
Western Blot: Collagen V Antibody (1E2-E4/Col5)BSA Free [NBP1-05118]
Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)Applications for Collagen V Antibody (1E2-E4/Col5) - BSA Free
Application
Recommended Usage
ELISA
1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-05118 can be used for detection of collagens by ELISA. NBP1-05118 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB: In immunoblotting NBP1-05118 detects human type V collagen only in its native triple helix form.
IHC: NBP1-05118 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Collagen V
Alternate Names
alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1
Gene Symbol
COL5A1
Additional Collagen V Products
Product Documents for Collagen V Antibody (1E2-E4/Col5) - BSA Free
Product Specific Notices for Collagen V Antibody (1E2-E4/Col5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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