Connexin 26/GJB2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41304
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat, Porcine
Cited:
Rat
Predicted:
Mouse (94%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 16 amino acid peptide near the center of human GJB2. The immunogen is located within amino acids 80 - 130 of GJB2. Amino Acid Squence: HVAYRRHEKKRKFIKGE
Reactivity Notes
Predicted species reactivity based on immunogen sequence: Sheep: (88%), Bovine: (88%), Chicken: (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Connexin 26/GJB2 Antibody - BSA Free
Western Blot: Connexin 26/GJB2 AntibodyBSA Free [NBP2-41304]
Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]
Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.Immunohistochemistry: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]
Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.Applications for Connexin 26/GJB2 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1 - 2 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33171391)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Connexin 26/GJB2
Alternate Names
CX26, DFNA3, DFNB1, GJB2, HID, KID, NSRD1, PPK
Gene Symbol
GJB2
Additional Connexin 26/GJB2 Products
Product Documents for Connexin 26/GJB2 Antibody - BSA Free
Product Specific Notices for Connexin 26/GJB2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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