Connexin 30/GJB6 Antibody (CL4540)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61141

Novus Biologicals, part of Bio-Techne
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NBP2-61141
NBP2-61141-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4540

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Connexin 30/GJB6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:RSKRAQTQKNHPNHALKESK

Reactivity Notes

Mouse, Rat (85%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Connexin 30/GJB6 Antibody (CL4540)

Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]

Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]

Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of MCF7 cells using the Anti-GJB6 monoclonal antibody, showing no specific staining of cell junctions. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]

Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]

Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of RT-4 cells using the Anti-GJB6 monoclonal antibody, showing specific staining of cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Applications for Connexin 30/GJB6 Antibody (CL4540)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

Reactivity reported in (PMID: 31205407)
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Connexin 30/GJB6

The connexin gene family codes for the protein subunits of gap junction channels that mediate direct diffusion of ions and metabolites between the cytoplasm of adjacent cells. Connexins span the plasma membrane 4 times, with amino- and carboxy-terminal regions facing the cytoplasm. Connexin genes are expressed in a cell type-specific manner with overlapping specificity. The gap junction channels have unique properties depending on the type of connexins constituting the channel.[supplied by OMIM]

Alternate Names

CX30, DFNA3B, DFNB1B, ED2, EDH, GJB6

Gene Symbol

GJB6

Additional Connexin 30/GJB6 Products

Product Documents for Connexin 30/GJB6 Antibody (CL4540)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Connexin 30/GJB6 Antibody (CL4540)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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