Connexin 30/GJB6 Antibody (CL4540)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61141
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL4540
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:RSKRAQTQKNHPNHALKESK
Reactivity Notes
Mouse, Rat (85%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Connexin 30/GJB6 Antibody (CL4540)
![Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]](https://resources.bio-techne.com/images/products/Connexin-30-GJB6-Antibody-CL4540-Immunocytochemistry-Immunofluorescence-NBP2-61141-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]")
Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]
Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of MCF7 cells using the Anti-GJB6 monoclonal antibody, showing no specific staining of cell junctions. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.![Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]](https://resources.bio-techne.com/images/products/Connexin-30-GJB6-Antibody-CL4540-Immunocytochemistry-Immunofluorescence-NBP2-61141-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]")
Immunocytochemistry/ Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141]
Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of RT-4 cells using the Anti-GJB6 monoclonal antibody, showing specific staining of cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.Applications for Connexin 30/GJB6 Antibody (CL4540)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
Reactivity reported in (PMID: 31205407)
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Connexin 30/GJB6
Alternate Names
CX30, DFNA3B, DFNB1B, ED2, EDH, GJB6
Gene Symbol
GJB6
Additional Connexin 30/GJB6 Products
Product Documents for Connexin 30/GJB6 Antibody (CL4540)
Product Specific Notices for Connexin 30/GJB6 Antibody (CL4540)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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