Connexin 30.3/GJB4 Antibody (1E3-1C12)
Novus Biologicals, part of Bio-Techne | Catalog # H00127534-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E3-1C12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GJB4 (AAH34709, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNWAFLQGLLSGVNKYSTVLSRIWLSVVFIFRVLVYVVAAEEVWDDEQKDFVCNTKQPGCPNVCYDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREERERKHHLKHGPNAPSLYDNLSKKRGGLWWTYLLSLIFKAAVDAGFLYIFHRLYKDYDMPRVVACSVEPCPHTVDCYISRPTEKKVFTYFMVTTAAICILLNLSEVFYLVGKRCMEIFGPRHRRPRCRECLPDTCPPYVLSQGGHPEDGNSVLMKAGSAPVDAGGYP
Specificity
GJB4 - gap junction protein, beta 4 (connexin 30.3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Connexin 30.3/GJB4 Antibody (1E3-1C12)
![Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]](https://resources.bio-techne.com/images/products/Connexin-30-3-GJB4-Antibody-1E3-1C12-Western-Blot-H00127534-M01-img0005.jpg "Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]")
Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]
Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - GJB4 monoclonal antibody (M01), clone 1E3-1C12 Analysis of GJB4 expression in Hela.![Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]](https://resources.bio-techne.com/images/products/Connexin-30-3-GJB4-Antibody-1E3-1C12-Western-Blot-H00127534-M01-img0006.jpg "Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]")
Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]
Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Analysis of GJB4 expression in transfected 293T cell line by GJB4 monoclonal antibody (M01), clone 1E3-1C12.Lane 1: GJB4 transfected lysate(30 KDa).Lane 2: Non-transfected lysate.![ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]](https://resources.bio-techne.com/images/products/Connexin-30-3-GJB4-Antibody-1E3-1C12-ELISA-H00127534-M01-img0007.jpg "ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]")
ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]
ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Detection limit for recombinant GST tagged GJB4 is approximately 3ng/ml as a capture antibody.Applications for Connexin 30.3/GJB4 Antibody (1E3-1C12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Connexin 30.3/GJB4
Alternate Names
CX30.3, GJB4
Entrez Gene IDs
127534 (Human)
Gene Symbol
GJB4
OMIM
133 (Human)
UniProt
Additional Connexin 30.3/GJB4 Products
Product Documents for Connexin 30.3/GJB4 Antibody (1E3-1C12)
Product Specific Notices for Connexin 30.3/GJB4 Antibody (1E3-1C12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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