Connexin 43/GJA1 [p Ser368] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-722
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Guinea Pig (100%), Human (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH. Accession # P08050
Modification
p Ser368
Localization
Cytoplasmic and Integral membrane protein
Specificity
Specific for endogenous levels of the ~43 kDa Connexin 43/GJA1 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Marker
Gap Junctions Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Connexin 43/GJA1 [p Ser368] Antibody
![Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722]](https://resources.bio-techne.com/images/products/Connexin-43-GJA1-[p-Ser368]-Antibody-Western-Blot-NB100-722-img0001.jpg "Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722]")
Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722]
Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.Applications for Connexin 43/GJA1 [p Ser368] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~43 kDa.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Connexin 43/GJA1
Alternate Names
CX43, GJA1, GJAL, ODDD, ODOD, SDTY3
Gene Symbol
GJA1
UniProt
Additional Connexin 43/GJA1 Products
Product Documents for Connexin 43/GJA1 [p Ser368] Antibody
Product Specific Notices for Connexin 43/GJA1 [p Ser368] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...