COP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-856

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-856
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all COP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ETLGLSAGPIPGN corresponding to C-Terminus according to NP_443121.1.

Specificity

This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COP Antibody

Immunocytochemistry/ Immunofluorescence: COP Antibody [NB100-856]

Immunocytochemistry/ Immunofluorescence: COP Antibody [NB100-856]

Immunocytochemistry/Immunofluorescence: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: COP Antibody [NB100-856]

Flow Cytometry: COP Antibody [NB100-856]

Flow Cytometry: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for COP Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 28-30 kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7 kDa band according to NP_443121.1. The 28-30 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COP

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of theproteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release ofIL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokineresponse. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, andstaurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspasesCASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2

Alternate Names

CARD only domain-containing protein 1, CARD only protein, caspase recruitment domain family, member 16, Caspase-1 inhibitor COP, COP1caspase-1 dominant-negative inhibitor pseudo-ICE, COPcaspase recruitment domain-containing protein 16, EC 3.4.22.36, Pseudo interleukin-1 beta converting enzyme, pseudo interleukin-1beta converting enzyme, Pseudo-ICE

Entrez Gene IDs

114769 (Human)

Gene Symbol

CARD16

UniProt

NP_443121. (Human)

Additional COP Products

Product Documents for COP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for COP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.