COPG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55178

Novus Biologicals, part of Bio-Techne
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NBP2-55178
NBP2-55178-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COPG Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MLPSILVLLKRCVMDDDNEVRDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLERALQQYTLEPSEKPFDLKSVPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COPG Antibody

Western Blot: COPG Antibody [NBP2-55178]

Western Blot: COPG Antibody [NBP2-55178]

Western Blot: COPG Antibody [NBP2-55178] - Analysis using Anti-COPG1 antibody NBP2-55178 (A) shows similar pattern to independent antibody NBP1-85514 (B).
Immunocytochemistry/ Immunofluorescence: COPG Antibody [NBP2-55178]

Immunocytochemistry/ Immunofluorescence: COPG Antibody [NBP2-55178]

Immunocytochemistry/Immunofluorescence: COPG Antibody [NBP2-55178] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: COPG Antibody [NBP2-55178]

Immunohistochemistry-Paraffin: COPG Antibody [NBP2-55178]

Immunohistochemistry-Paraffin: COPG Antibody [NBP2-55178] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Applications for COPG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COPG

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors

Alternate Names

coat protein gamma-cop, coatomer protein complex, subunit gamma, coatomer protein complex, subunit gamma 1, COPG1coatomer subunit gamma, FLJ21068, Gamma-coat protein, Gamma-COP

Gene Symbol

COPG1

Additional COPG Products

Product Documents for COPG Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for COPG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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