Coronin-1a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58867

Novus Biologicals, part of Bio-Techne
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NB110-58867
NB110-58867SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Coronin-1a Antibodies »

Product Specifications

Immunogen

C-terminal peptide of human coronin 1a coupled to KLH. [UniProt# P31146]

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coronin-1a Antibody

Western Blot: Coronin-1a Antibody [NB110-58867]

Western Blot: Coronin-1a Antibody [NB110-58867]

Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.
Immunocytochemistry/ Immunofluorescence: Coronin-1a Antibody [NB110-58867]

Immunocytochemistry/ Immunofluorescence: Coronin-1a Antibody [NB110-58867]

Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58867] - Analysis of cortical neuron-glial cell culture from E20 rat stained with Coronin-1a Antibody, dilution 1:1,000 (Red), and costained with mouse mAb to GFAP, dilution 1:1,000 (Green). Hoechst staining of nuclear DNA (Blue). The Coronin-1a Antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.

Applications for Coronin-1a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Western Blot

1:10000
Application Notes
This Coronin-1a antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coronin-1a

Highly conserved coronin family of proteins/actin regulators from WD-repeat superfamily were originally identified in Dictyostelium, wherein, coronin deletions were observed to affect actin-dependent processes, such as phagocytosis, cell motility, and cytokinesis. Coronin-1a (Coro1A) belongs to Type I coronins, almost exclusively expressed in hematopoietic lineages, and plays a key role during T lymphocyte activation, migration, survival, and calcium signaling. Moreover, Coronin-1a has been documented to be dispensable for actin-dependent functions in macrophages. Coro1a-deficient mice have reduced peripheral T cells due to both an impaired survival of naive cells and migrational defects. Both CD4+ and CD8+ T cell subsets are decreased in the periphery of Coro1a-/- mice while thymic cellularity and subpopulations are similar to those observed in wild-type mice. A nonsense mutation in Coro1a has been shown to protect against systemic lupus in a mouse model. Moreover, it has shown that Coro1a is mutated in a thymic egress-deficient mouse strain, but also in a patient with severe combined immunodeficiency (SCID).

Alternate Names

CLIPINA, Clipin-A, CORO1, coronin, actin binding protein, 1A, coronin, actin-binding protein, 1A, coronin-1, coronin-1A, Coronin-like protein A, Coronin-like protein p57, FLJ41407, HCORO1, MGC117380, p57, TACOCLABP, Tryptophan aspartate-containing coat protein

Entrez Gene IDs

11151 (Human)

Gene Symbol

CORO1A

UniProt

P31146 (Human)

Additional Coronin-1a Products

Product Documents for Coronin-1a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Coronin-1a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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