COX-2 Antibody (10B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26361
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for COX-2 Antibody (10B2)
Immunogen
A synthesized peptide derived from Human COX-2 [UniProt P35354]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COX-2 Antibody (10B2)
Western Blot: COX-2 Antibody (10B2) [NBP3-26361] -
Western Blot: COX-2 Antibody (10B2) [NBP3-26361] - Positive Western Blot detected in: U-87 whole cell lysate.All lanes: COX2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Immunocytochemistry/Immunofluorescence: COX-2 Antibody (10B2) [NBP3-26361] -
Immunocytochemistry/Immunofluorescence: COX-2 Antibody (10B2) [NBP3-26361] - Staining of HepG2 Cells with COX-2 Antibody (10B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for COX-2 Antibody (10B2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: COX-2
Long Name
Cyclooxygenase 2
Alternate Names
COX2, PGHS-2, PHS-II, PTGS2
Gene Symbol
PTGS2
Additional COX-2 Products
Product Documents for COX-2 Antibody (10B2)
Product Specific Notices for COX-2 Antibody (10B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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