COX-2 Antibody (2V7G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16220
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2V7G8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 505-604 of human COX-2 (P35354). GETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for COX-2 Antibody (2V7G8)
![Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220]](https://resources.bio-techne.com/images/products/COX-2-Antibody-2V7G8-Western-Blot-NBP3-16220-img0002.jpg "Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220]")
Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220]
Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -
Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -
Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for COX-2 Antibody (2V7G8)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: COX-2
Long Name
Cyclooxygenase 2
Alternate Names
COX2, PGHS-2, PHS-II, PTGS2
Gene Symbol
PTGS2
Additional COX-2 Products
Product Documents for COX-2 Antibody (2V7G8)
Product Specific Notices for COX-2 Antibody (2V7G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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