COX-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-689

Novus Biologicals, part of Bio-Techne
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NB100-689
NB100-689SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all COX-2 Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).

Localization

Microsomal membrane, cytoplasmic

Specificity

This reacts with the 70 kDa protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COX-2 Antibody

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).
Simple Western: COX-2 Antibody [NB100-689]

Simple Western: COX-2 Antibody [NB100-689]

Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.

Applications for COX-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Simple Western

1:100

Western Blot

1:100-1:2000
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31592103).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 2 using NB100-689 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Entrez Gene IDs

5743 (Human); 19225 (Mouse); 29527 (Rat)

Gene Symbol

PTGS2

UniProt

P35355 (Rat)

Additional COX-2 Products

Product Documents for COX-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for COX-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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