COX IV Isoform 2 Antibody (1F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00084701-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunoblotting, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COX4-I2 Antibodies »

Product Specifications

Immunogen

COX4I2 (NP_115998, 21 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MHSSEGTTRGGGKMSPYTNCYAQRYYPMPEEPFCTELNAEEQALKEKEKGSWTQLTHAEKVALYRLQFNETFAEMNRRSNEWKT

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22730437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

COX4I2 - cytochrome c oxidase subunit IV isoform 2 (lung)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for COX IV Isoform 2 Antibody (1F2)

Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 monoclonal antibody (M01), clone 1F2.Lane 1: COX4I2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of monoclonal antibody to COX4I2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01]

Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 transfected lysate using anti-COX4I2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COX4I2 MaxPab rabbit polyclonal antibody.
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Applications for COX IV Isoform 2 Antibody (1F2)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COX4-I2

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation.

Long Name

Cytochrome c Oxidase Subunit IV Isoform 2

Alternate Names

COX4-2, COX4B, COX4L2, COXIV-2

Entrez Gene IDs

84701 (Human)

Gene Symbol

COX4I2

OMIM

607976 (Human)

UniProt

NP_115998 (Human)

Additional COX4-I2 Products

Product Documents for COX IV Isoform 2 Antibody (1F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COX IV Isoform 2 Antibody (1F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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