COX4 [p Ser58] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29532

Novus Biologicals, part of Bio-Techne
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NBP2-29532
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all COX4 Antibodies »

Product Summary for COX4 [p Ser58] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Accession # P19783

Modification

p Ser58

Specificity

Specific for endogenous levels of the ~17 kDa COX4 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX4 [p Ser58] Antibody

Western Blot: COX4 [p Ser58] Antibody [NBP2-29532]

Western Blot: COX4 [p Ser58] Antibody [NBP2-29532]

Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Applications for COX4 [p Ser58] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COX4

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (Acin-Perez et al., 2011).

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Entrez Gene IDs

12857 (Mouse)

Gene Symbol

COX4I1

UniProt

P19783 (Mouse)

Additional COX4 Products

Product Documents for COX4 [p Ser58] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for COX4 [p Ser58] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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