CPT1A Antibody (PSH04-00)

Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213]

Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: SK-OV-3 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: Human kidney tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213]

Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213]

Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Flow cytometric analysis of HepG2 cells labeling CPT1A.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32213, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).