CPT1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-53791
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DPAQTVEQRLKLFK corresponding to internal region according to NP_001867.2, NP_001027017.1.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25026599). Rat reactivity reported in scientific literature (PMID: 23045433).
Specificity
This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CPT1A Antibody
![Western Blot: CPT1A Antibody [NB100-53791]](https://resources.bio-techne.com/images/products/CPT1A-Antibody-Western-Blot-NB100-53791-img0002.jpg "Western Blot: CPT1A Antibody [NB100-53791]")
Western Blot: CPT1A Antibody [NB100-53791]
Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.![Western Blot: CPT1A Antibody [NB100-53791]](https://resources.bio-techne.com/images/products/CPT1A-Antibody-Western-Blot-NB100-53791-img0001.jpg "Western Blot: CPT1A Antibody [NB100-53791]")
Western Blot: CPT1A Antibody [NB100-53791]
Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] -
Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.Applications for CPT1A Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5-7 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
1-3 ug/mL
Application Notes
Western blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2 kDa according to NP_001027017.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CPT1A
Long Name
Carnitine O-palmitoyltransferase 1, liver isoform
Alternate Names
CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21
Entrez Gene IDs
1374 (Human)
Gene Symbol
CPT1A
UniProt
Additional CPT1A Products
Product Documents for CPT1A Antibody
Product Specific Notices for CPT1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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