CRABP1 Antibody (7N4F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16601

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16601-100ul
NBP3-16601-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7N4F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRABP1 Antibodies »

Product Summary for CRABP1 Antibody (7N4F3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 58-137 of human CRABP1 (P29762). TVRTTEINFKVGEGFEEETVDGRKCRSLATWENENKIHCTQTLLEGDGPKTYWTRELANDELILTFGADDVVCTRIYVRE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRABP1 Antibody (7N4F3)

Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601]

Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601]

Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601] - Analysis of extracts of various cell lines, using CRABP1 Rabbit mAb (NBP3-16601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry: CRABP1 Antibody (7N4F3) [NBP3-16601]

Immunohistochemistry: CRABP1 Antibody (7N4F3) [NBP3-16601]

Immunohistochemistry: CRABP1 Antibody (7N4F3) [NBP3-16601] - Immunofluorescence analysis of rat eye using CRABP1 Rabbit mAb (NBP3-16601) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601]

Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601]

Western Blot: CRABP1 Antibody (7N4F3) [NBP3-16601] - Analysis of extracts of Mouse testis, using CRABP1 Rabbit mAb (NBP3-16601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for CRABP1 Antibody (7N4F3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CRABP1

Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The Cellular Retinoic Acid Binding Proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins. Two forms of CRABP have been characterized, CRABP 1 and CRABP 2 which share ~75% sequence conservation. The main role of CRABP 1 may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABP 2 is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).

Alternate Names

cellular retinoic acid binding protein 1, CRABP, CRABPI, CRABP-Icellular retinoic acid-binding protein 1, RBP5Cellular retinoic acid-binding protein I

Gene Symbol

CRABP1

Additional CRABP1 Products

Product Documents for CRABP1 Antibody (7N4F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CRABP1 Antibody (7N4F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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