CRABP2 Antibody (JE65-66)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32214
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-66
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human CRABP2 aa 1-138/138. (Uniprot: P29373)
Localization
Endoplasmic reticulum, Nucleus, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CRABP2 Antibody (JE65-66)
Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214]
Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214] - Western blot analysis of CRABP2 on MCF-7 cell lysates with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/1,000 dilution.Lysates/proteins at 10 ug/Lane.
Predicted band size: 16 kDa
Observed band size: 14 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32214) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: CRABP2 Antibody (JE65-66) [NBP3-32214]
Immunohistochemistry: CRABP2 Antibody (JE65-66) [NBP3-32214] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/400 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32214) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for CRABP2 Antibody (JE65-66)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:400
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CRABP2
Long Name
Cellular Retinoic Acid Binding Protein 2
Alternate Names
CRABP-II, RBP6
Gene Symbol
CRABP2
Additional CRABP2 Products
Product Documents for CRABP2 Antibody (JE65-66)
Product Specific Notices for CRABP2 Antibody (JE65-66)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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