Can you please tell me if this antibody works for mouse?

In regards to your inquiry, we have not yet tested this antibody on mouse samples. I ran an alignment against the antigen sequence (VRKLREKRQTEGTYRPSSEEQFSHAAEARAPQDSKETVQGCLPI) with the mouse information on UniProt and found that it has very high similarity with Isoform 2. There is a chance this will work in mouse samples. If you would like to try this antibody in mouse samples, you can qualify for our Innovators Reward Program.

I am planning to use CRB3 antibody (NBP1-81185) for my bovine samples for IF. Has the antibody ever been tested with bovine samples and what is the sequence indenty of the epitope sequence with respect to bovine species?

Our product NBP1-81185 has not yet been tested for detection of the bovine protein. I was also not able to find the bovine CRB3 protein to run an alignment against, so I do not have any homology data available.

CRB3 Antibody

Name: CRB3 Antibody
Brand: Novus Biologicals
SKU: NBP1-81185

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81185

Submit a review

Citations (6)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRB3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRKLREKRQTEGTYRPSSEEQFSHAAEARAPQDSKETVQGCLPI

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29155075).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRB3 Antibody

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185]

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185] - Analysis in human kidney and cerebral cortex tissues using NBP1-81185 antibody. Corresponding CRB3 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence: CRB3 Antibody [NBP1-81185]

Immunocytochemistry/Immunofluorescence: CRB3 Antibody [NBP1-81185] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185]

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185] - Staining of human cerebral cortex shows low expression as expected.

View 1 more images

Applications for CRB3 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Immunoprecipitation

Reported in scientific literature (PMID:34404733)

Western Blot

Reported in scientific literature (PMID:34404733)

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRB3

The CRB3 gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding dif

Alternate Names

crumbs 3, crumbs homolog 3 (Drosophila), crumbs protein homolog 3, MGC17303

Entrez Gene IDs

92359 (Human)

Gene Symbol

CRB3

UniProt

Q9BUF7 (Human)

Additional CRB3 Products

Product Documents for CRB3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CRB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

CRB3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81185

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CRB3 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for CRB3 Antibody

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185]

Immunocytochemistry/ Immunofluorescence: CRB3 Antibody [NBP1-81185]

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185]

Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185]

Applications for CRB3 Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CRB3

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CRB3 Products

Product Documents for CRB3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CRB3 Antibody