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CRBN Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91810

Novus Biologicals, part of Bio-Techne
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NBP1-91810
NBP1-91810-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Knockout Validated, Simple Western, Single Cell Western, Western Blot

Cited:

Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQERE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRBN Antibody - BSA Free

Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CRBN Antibody [NBP1-91810]

Immunocytochemistry/ Immunofluorescence: CRBN Antibody [NBP1-91810]

Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.
Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for CRBN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Knockout Validated

Validation (PMID: 31371704).

Simple Western

1:60

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT4, U-251MG, separated by Size, antibody dilution of 1:60, apparent MW was 60 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 4 using NBP1-91810 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRBN

Modulates cell surface expression of KCNT1 . May be involved in memory and learning

Long Name

Cereblon

Alternate Names

Cereblon, MRT2, MRT2A, Protein X 0001

Entrez Gene IDs

51185 (Human)

Gene Symbol

CRBN

UniProt

Additional CRBN Products

Product Documents for CRBN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CRBN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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