Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59601
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2ba6
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
Immunogen
Human Creatine Kinase BB protein (Uniprot: P12277)
Reactivity Notes
Predicted to react with Chimpanzee, Rhesus Monkey, Canine, Bovine, Mouse, Rat, Chicken, Zebrafish, Frog.
Localization
Cytoplasmic
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This monoclonal antibody recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59600).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
Protein Array: Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free [NBP2-59601]
Protein Array: Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free [NBP2-59601] - Analysis of Protein Array containing more than 19,000 containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of Ab to its intended target.Applications for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen Only): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Frozen Only): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
Product Specific Notices for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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