Creatine Kinase BB Antibody (8E4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59462
![Western Blot: Creatine Kinase BB Antibody (8E4)BSA Free [NBP2-59462]](https://resources.bio-techne.com/images/products/Creatine-Kinase-B-Antibody-8E4-Western-Blot-NBP2-59462-img0001.jpg "Western Blot: Creatine Kinase BB Antibody (8E4)BSA Free [NBP2-59462]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8E4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Creatine Kinase BB (1-381aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Creatine Kinase BB Antibody (8E4) - BSA Free
Western Blot: Creatine Kinase BB Antibody (8E4)BSA Free [NBP2-59462]
Western Blot: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Lane 1: HeLa cell lysates, Lane 2: 293T cell lysates, Lane 2: K562 cell lysates, Lane 2: LnCap cell lysates![Immunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]](https://resources.bio-techne.com/images/products/Creatine-Kinase-B-Antibody-8E4-Immunocytochemistry-Immunofluorescence-NBP2-59462-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]")
Immunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]
Immunocytochemistry/Immunofluorescence: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Flow Cytometry: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]](https://resources.bio-techne.com/images/products/Creatine-Kinase-B-Antibody-8E4-Flow-Cytometry-NBP2-59462-img0003.jpg "Flow Cytometry: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]")
Flow Cytometry: Creatine Kinase BB Antibody (8E4) - BSA Free [NBP2-59462]
Flow Cytometry: Creatine Kinase B Antibody (8E4) [NBP2-59462] - Analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for Creatine Kinase BB Antibody (8E4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1063
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (8E4) - BSA Free
Product Specific Notices for Creatine Kinase BB Antibody (8E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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