Creatine Kinase BB Antibody (CPTC-CKB-2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07542
![Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542]](https://resources.bio-techne.com/images/products/Creatine-Kinase-BB-Antibody-CPTC-CKB-2-Western-Blot-NBP3-07542-img0001.jpg "Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # CPTC-CKB-2
Concentration
0.2 mg/ml
Product Summary for Creatine Kinase BB Antibody (CPTC-CKB-2)
Immunogen
Recombinant human full-length Creatine Kinase BB protein (Uniprot: P12277)
Localization
Cytoplasm
Specificity
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This monoclonal antibody recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08866)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Creatine Kinase BB Antibody (CPTC-CKB-2)
Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542]
Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542] - Western Blot Analysis of HEK293 cell lysate using Creatine Kinase BB Mouse Monoclonal Antibody (CPTC-CKB-2).Applications for Creatine Kinase BB Antibody (CPTC-CKB-2)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Creatine Kinase BB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (CPTC-CKB-2)
Product Specific Notices for Creatine Kinase BB Antibody (CPTC-CKB-2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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