Creatine Kinase BB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2847

Novus Biologicals, part of Bio-Techne
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NB100-2847
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Creatine Kinase BB Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KPSDEHKTDLNPDN corresponding to internal region according to NP_001814.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Creatine Kinase BB Antibody

Western Blot: Creatine Kinase BB Antibody [NB100-2847]

Western Blot: Creatine Kinase BB Antibody [NB100-2847]

Western Blot: Creatine Kinase B Antibody [NB100-2847] - Analysis using NB100-2847 at 0.1 ug/ml in Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Creatine Kinase BB Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 45-48 kDa band observed in human brain lysates (calculated MW of 42.6 kDa band according to NP_001814.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Creatine Kinase BB

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Alternate Names

B-CK, CKB

Entrez Gene IDs

1152 (Human); 12709 (Mouse)

Gene Symbol

CKB

UniProt

NP_001814.2 (Human)

Additional Creatine Kinase BB Products

Product Documents for Creatine Kinase BB Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Creatine Kinase BB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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