Creatine Kinase BB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84460
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Creatine Kinase BB Antibody
Immunocytochemistry/ Immunofluorescence: Creatine Kinase BB Antibody [NBP1-84460]
Immunocytochemistry/Immunofluorescence: Creatine Kinase BB Antibody [NBP1-84460] - Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460]
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody [NBP1-84460] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.Applications for Creatine Kinase BB Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
Reported in scientific literature (PMID: 26460485)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody
Product Specific Notices for Creatine Kinase BB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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