Creatine Kinase BB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00288

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Creatine Kinase BB Antibodies »

Product Summary for Creatine Kinase BB Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human Creatine Kinase BB (Accession#: P12277; Pro2-Lys381)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Creatine Kinase BB Antibody

Western Blot: Creatine Kinase BB Antibody [NBP3-00288]

Western Blot: Creatine Kinase BB Antibody [NBP3-00288]

Western Blot: Creatine Kinase BB Antibody [NBP3-00288] - Anti-Creatine Kinase BB rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa
Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288]

Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288]

Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288] - Creatine Kinase BB was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-Creatine Kinase BB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Creatine Kinase BB rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa

Applications for Creatine Kinase BB Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Creatine Kinase BB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Creatine Kinase BB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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