Creatine Kinase, Muscle/CKMM Antibody (1E3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00001158-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1E3
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CKM (NP_001815, 282 a.a. ~ 381 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
Specificity
CKM - creatine kinase, muscle
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Creatine Kinase, Muscle/CKMM Antibody (1E3) - Azide and BSA Free
![Western Blot: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]](https://resources.bio-techne.com/images/products/Creatine-Kinase-Muscle-CKMM-Antibody-1E3-Western-Blot-H00001158-M02-img0006.jpg "Western Blot: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]")
Western Blot: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]
Western Blot: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - CKM monoclonal antibody (M02), clone 1E3 Analysis of CKM expression in IMR-32.![Immunocytochemistry/ Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]](https://resources.bio-techne.com/images/products/Creatine-Kinase-Muscle-CKMM-Antibody-1E3-Immunocytochemistry-Immunofluorescence-H00001158-M02-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]")
Immunocytochemistry/ Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]
Immunocytochemistry/Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Analysis of monoclonal antibody to CKM on HeLa cell . Antibody concentration 10 ug/ml.![ELISA: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]](https://resources.bio-techne.com/images/products/Creatine-Kinase-Muscle-CKMM-Antibody-1E3-ELISA-H00001158-M02-img0007.jpg "ELISA: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]")
ELISA: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02]
ELISA: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Detection limit for recombinant GST tagged CKM is 0.03 ng/ml as a capture antibody.Applications for Creatine Kinase, Muscle/CKMM Antibody (1E3) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Creatine Kinase, Muscle/CKMM
Long Name
Creatine Kinase, Muscle
Alternate Names
CKM, CKMM, CKMMcreatine kinase M-type, Creatine Kinase Isoenzyme, Creatine kinase M chain, creatine kinase, muscle, creatine kinase-M, EC 2.7.3, EC 2.7.3.2, M-CK
Entrez Gene IDs
1158 (Human)
Gene Symbol
CKM
OMIM
123310 (Human)
UniProt
Additional Creatine Kinase, Muscle/CKMM Products
Product Documents for Creatine Kinase, Muscle/CKMM Antibody (1E3) - Azide and BSA Free
Product Specific Notices for Creatine Kinase, Muscle/CKMM Antibody (1E3) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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