CREB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-87001

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-87001
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CREB Antibodies »

Product Summary for CREB Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human cAMP responsive element binding protein 1 using the numbering given in entry NP_604391.1 (GeneID 1385).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CREB Antibody

Western Blot: CREB Antibody [NB100-87001]

Western Blot: CREB Antibody [NB100-87001]

Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001]

Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001]

Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CREB used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for CREB Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CREB

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

UniProt

P16220

Additional CREB Products

Product Documents for CREB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CREB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.