CREB [p Ser133] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-273

Novus Biologicals, part of Bio-Techne
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NB300-273
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CREB Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH. Accession # P15337

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34346292). Mouse reactivity reported in scientific literature (PMID: 21838783). .

Modification

p Ser133

Localization

Nuclear

Specificity

Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CREB [p Ser133] Antibody

Western Blot: CREB [p Ser133] Antibody [NB300-273]

Western Blot: CREB [p Ser133] Antibody [NB300-273]

Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).
Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273]

Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273]

Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.

Applications for CREB [p Ser133] Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000
Application Notes
This antibody has not been validated for use in paraffin embedded sections (IHC-P). For IHC-Fr, the antibody was used at 1:1000 and incubated for 48 hours at 4C. The dilution buffer used was 0.1M PBS, 0.5% Triton-100, and 3% normal serum. No antigen retrieval step was performed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CREB

It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogenactivated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs)phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1, pCREB

Entrez Gene IDs

1385 (Human); 81646 (Rat)

Gene Symbol

CREB1

UniProt

P15337 (Rat)

Additional CREB Products

Product Documents for CREB [p Ser133] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CREB [p Ser133] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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