CRHR1/CRF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00175
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NEEKKSKVHYH corresponding to internal region according to NP_004373.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CRHR1/CRF1 Antibody
![Western Blot: CRHR1/CRF1 Antibody [NBP1-00175]](https://resources.bio-techne.com/images/products/CRHR1-CRF1-Antibody-Western-Blot-NBP1-00175-img0005.jpg "Western Blot: CRHR1/CRF1 Antibody [NBP1-00175]")
Western Blot: CRHR1/CRF1 Antibody [NBP1-00175]
Western Blot: CRHR1/CRF1 Antibody [NBP1-00175] - Staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]](https://resources.bio-techne.com/images/products/CRHR1-CRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00175-img0007.jpg "Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]")
Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]
Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]](https://resources.bio-techne.com/images/products/CRHR1-CRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00175-img0006.jpg "Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]")
Immunocytochemistry/ Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175]
Immunocytochemistry/Immunofluorescence: CRHR1/CRF1 Antibody [NBP1-00175] - Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, and vesicle staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for CRHR1/CRF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:128000
Western Blot
0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). Primary incubation was 1 hour. IHC reported in scientific literature (PMID: 22570383). Use in ICC/IF reported in scientific literature (PMID: 30439403).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CRHR1
Long Name
Corticotropin Releasing Hormone Receptor 1
Alternate Names
CHR-R1, CRF1, CRFR, CRFR1
Gene Symbol
CRHR1
UniProt
Additional CRHR1 Products
Product Documents for CRHR1/CRF1 Antibody
Product Specific Notices for CRHR1/CRF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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