CRHR2/CRF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56485

Novus Biologicals, part of Bio-Techne
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NB100-56485
NB100-56485SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Mammal

Cited:

Mouse, Rat, Mammal

Predicted:

Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CRHR2 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 75-125 of human CRHR2 was used as the immunogen.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID:33344730) 94% sequence identity with Rhesus monkey

Specificity

There are three isoforms of CRHR2 in human: alpha is 411 amino acids, beta is 438 amino acids, and gamma is 397 amino acids.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRHR2/CRF2 Antibody

Western Blot: CRHR2/CRF2 Antibody [NB100-56485]

Western Blot: CRHR2/CRF2 Antibody [NB100-56485]

Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.
Simple Western: CRHR2/CRF2 Antibody [NB100-56485]

Simple Western: CRHR2/CRF2 Antibody [NB100-56485]

Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for CRHR2/CRF2 Antibody

Application
Recommended Usage

Simple Western

1:200

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56485 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRHR2

CRHR2 is a novel member of Secretin-family-GPCR proteins that functions as a functional receptor for CRF. It is expressed as 3 isoforms; alpha, beta and gamma. These isoforms primarily differ in their N-terminal sequence and tissue distribution. Apart from CRF, other ligands of CRHR2 include UcnI, UcnII and UcnIII. CRHR2 is involved in stress responses, cardiovascular function and gastric motility. Studies on CRHR2 deficient mice suggested a central anxiolytic effect of CRHR2. Northern Blot analysis detects CRHR2 expression in brain, hypothalamus, heart, GI, lung, skeletal muscle and vasculature.

Long Name

Corticotropin Releasing Hormone Receptor 2

Alternate Names

CRF-RB, CRF2, HM-CRF

Entrez Gene IDs

1395 (Human); 12922 (Mouse); 64680 (Rat)

Gene Symbol

CRHR2

UniProt

NP_001874 (Human)

Additional CRHR2 Products

Product Documents for CRHR2/CRF2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CRHR2/CRF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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