CRIPTO Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1598

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of mouse Cripto1 (between residues 1-50). [UniProt# P51865]

Localization

Membrane-bound

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRIPTO Antibody - BSA Free

Western Blot: CRIPTO AntibodyBSA Free [NB100-1598]

Western Blot: CRIPTO AntibodyBSA Free [NB100-1598]

Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody - BSA Free [NB100-1598]

Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody - BSA Free [NB100-1598]

Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow Cytometry: CRIPTO Antibody - BSA Free [NB100-1598]

Flow Cytometry: CRIPTO Antibody - BSA Free [NB100-1598]

Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
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Applications for CRIPTO Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

2 ug/ml
Application Notes
This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cripto

Cripto-1 (teratocarcinoma-derived growth factor-1) is the original member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate signaling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate signaling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, postnatally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.

Long Name

Teratocarcinoma-derived Growth Factor/Cripto Growth Factor

Alternate Names

CFC-2, TDGF-1, TDGF1

Gene Symbol

CRIPTO

Additional Cripto Products

Product Documents for CRIPTO Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CRIPTO Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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