Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRMP2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CRMP2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CRMP2 Antibody

Western Blot: CRMP2 Antibody [NBP2-97750]

Western Blot: CRMP2 Antibody [NBP2-97750]

Western Blot: CRMP2 Antibody [NBP2-97750] - Anti-CRMP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa
Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody [NBP2-97750]

Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody [NBP2-97750]

Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP2-97750] - Immunofluorescence staining of CRMP2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CRMP2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: CRMP2 Antibody [NBP2-97750] -

Immunoprecipitation: CRMP2 Antibody [NBP2-97750] - CRMP2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-CRMP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CRMP2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa

Applications for CRMP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRMP2

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Alternate Names

Collapsin response mediator protein 2, CRMP-2, CRMP2N2A3, DHPRP2, dihydropyrimidinase-like 2, dihydropyrimidinase-like 2 long form, dihydropyrimidinase-related protein 2, DRP2collapsin response mediator protein hCRMP-2, DRP-2ULIP-2, ULIP2, Unc-33-like phosphoprotein 2

Gene Symbol

DPYSL2

Additional CRMP2 Products

Product Documents for CRMP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CRMP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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