CRTAC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88864

Novus Biologicals, part of Bio-Techne
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NBP1-88864
NBP1-88864-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRTAC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDGKMVSRNVASGEMNSVLEILYPRDEDTLQDPAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRTAC1 Antibody

Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864]

Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864]

Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864] - Staining in human lung and pancreas tissues using anti-CRTAC1 antibody. Corresponding CRTAC1 RNA-seq data are presented for the same tissues.
Western Blot: CRTAC1 Antibody [NBP1-88864]

Western Blot: CRTAC1 Antibody [NBP1-88864]

Western Blot: CRTAC1 Antibody [NBP1-88864] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human lung tissue.
Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864]

Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864]

Immunohistochemistry-Paraffin: CRTAC1 Antibody [NBP1-88864] - Staining of human lung shows high expression.
View 1 more images

Applications for CRTAC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95-105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46-437) and one EGF-like motif (aa 559-605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545-661. Over aa 28-661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Gene Symbol

CRTAC1

Additional CRTAC1 Products

Product Documents for CRTAC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRTAC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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