CRTH-2/GPR44 Antibody (54N3F8) [DyLight 350]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19014UV

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 54N3F8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRTH-2 Antibodies »

Product Specifications

Immunogen

A recombinant protein containing multiple external domains of GPR44 (CRTH2) was used as immunogen for the antibody. NP_004769

Specificity

GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. GPR44 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for CRTH-2/GPR44 Antibody (54N3F8) [DyLight 350]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CRTH-2

GPR44, also called CRTH2, is a Prostanoid Receptor that binds prostaglandin D2. PGD2 signals through CRTH2 to induce Th2, eosinophil, and basophil chemotaxis in a G-alpha(i) dependent manner. CRTH2 may play a role in mast cell-mediated immune responses such as those involved in allergic reactions. This receptor is upregulated in activated T helper-2 (Th2) cells, peripheral blood basophils, and eosinophils, but not in Th1 lineage cells. It may be a useful marker for Th2 and Tc2 cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated, a 3.5-kb transcript expressed primarily in the thalamus, frontal cortex, pons, and hippocampus, and a 3.4-kb transcript expressed in fetal liver, leukocytes, and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis, and thymus libraries.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Gene Symbol

PTGDR2

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody (54N3F8) [DyLight 350]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRTH-2/GPR44 Antibody (54N3F8) [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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