CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80682

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 54N3F8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CRTH-2 Antibodies »

Product Specifications

Immunogen

A recombinant protein containing multiple external domains of GPR44 (CRTH2) was used as immunogen for the antibody. NP_004769

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

Western Blot: CRTH-2/GPR44 Antibody (54N3F8)Azide and BSA Free [NBP2-80682]

Western Blot: CRTH-2/GPR44 Antibody (54N3F8)Azide and BSA Free [NBP2-80682]

Western Blot: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682] - Analysis of recombinant CRTH2 (GPR44) probed with CRTH2 antibody at 1 ug/ml. Image from the standard format of this antibody.
Immunohistochemistry: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682]

Immunohistochemistry: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682]

Immunohistochemistry: CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free [NBP2-80682] - Human testis stained with CRTH2 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.

Applications for CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRTH-2

GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. CRTH2 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells (1). CRTH2 is a prostaglandin D2 receptor PGD2 and has some similar functions to other chemokines exerting chemotaxis of Th2 cells as well as eosinophils and basophils (2). Interestingly inhibition of hair growth by PGD2 is through CRTH2 so the thought is that inhibition of CRTH2 may also eliminate hair-loss or balding effects of PGD2 . This implies that inhibitors to CRTH2 could be the new Rogaine(?) (3, 4). CRTH2 is thought to be important in allergic diseases, including asthma, and several companies are already testing drugs that block CRTH2 in clinical trials to treat these diseases (4).

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Gene Symbol

PTGDR2

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRTH-2/GPR44 Antibody (54N3F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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