CSK Antibody (5F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37651

Novus Biologicals, part of Bio-Techne
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NBP2-37651
NBP2-37651-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F3

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CSK Antibodies »

Product Summary for CSK Antibody (5F3) - BSA Free

Immunogen

Purified recombinant fragment of human CSK expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CSK Antibody (5F3) - BSA Free

Western Blot: CSK Antibody (5F3)BSA Free [NBP2-37651]

Western Blot: CSK Antibody (5F3)BSA Free [NBP2-37651]

Western Blot: CSK Antibody (5F3) [NBP2-37651] - Western blot analysis using CSK mouse mAb against NIH/3T3 (1),Hela (2),COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: CSK Antibody (5F3) - BSA Free [NBP2-37651]

Immunocytochemistry/ Immunofluorescence: CSK Antibody (5F3) - BSA Free [NBP2-37651]

Immunocytochemistry/Immunofluorescence: CSK Antibody (5F3) [NBP2-37651] - Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: CSK Antibody (5F3) - BSA Free [NBP2-37651]

Flow Cytometry: CSK Antibody (5F3) - BSA Free [NBP2-37651]

Flow Cytometry: CSK Antibody (5F3) [NBP2-37651] - Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).

Applications for CSK Antibody (5F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-37651 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CSK

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Gene Symbol

CSK

Additional CSK Products

Product Documents for CSK Antibody (5F3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSK Antibody (5F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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