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CSK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85951

Novus Biologicals, part of Bio-Techne
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NBP1-85951
NBP1-85951-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELH

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22665063)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CSK Antibody

Western Blot: CSK Antibody [NBP1-85951]

Western Blot: CSK Antibody [NBP1-85951]

Western Blot: CSK Antibody [NBP1-85951] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: CSK Antibody [NBP1-85951]

Immunocytochemistry/ Immunofluorescence: CSK Antibody [NBP1-85951]

Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP1-85951] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951]

Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951]

Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in lymphoid cells.

Applications for CSK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CSK

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Gene Symbol

CSK

Additional CSK Products

Product Documents for CSK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CSK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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