Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CTCF Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 150 and 200 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CTCF Antibody - BSA Free

Immunoprecipitation: CTCF Antibody [NB500-175]

Immunoprecipitation: CTCF Antibody [NB500-175]

Immunoprecipitation: CTCF Antibody [NB500-175] - Immunoprecipitation of Human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-175 used at 3 mcg/mg lysate for IP. Human CTCF was also immunoprecipitated using affinity purified rabbit anti-CTCF antibodies NB500-177 and NB500-194 CTCF was blotted using NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.

Applications for CTCF Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
NB 500-175 may be used for immunoprecipitation. Not recommended for Western Blot, use NB 500-177. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CTCF

The vertebrate insulator protein, CCCTC binding factor (CTCF) is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF contains 11 zinc finger motifs and it is thought that CTCF uses different combinations of zinc fingers to recognise a variety of specific sequences on the DNA to control several important genes. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is a negative regulator of cell proliferation and is located in a region of the chromosome that is frequently deleted in human cancers. The CTCF protein is altered in some breast and prostate tumours. Substitutions from one amino acid to another in the DNA-binding domain change the whole spectrum of the genes regulated by CTCF which can result in cancer development. CTCF was first identified as one of the nuclear factors binding to the regulatory regions of the c-myc gene. Further study of this protein revealed roles in transcriptional repression, insulator function, and imprinting genetic information. Among the multitude of normal CTCF functions, its involvement in partitioning the genome into active or inactive domains by means of a chromatin insulator function has received particular attention. As an insulator CTCF helps to define the boundaries between the neighbouring genes. Strikingly, all vertebrate chromatin insulators identified so far interact with CTCF.

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Entrez Gene IDs

10664 (Human)

Gene Symbol

CTCF

UniProt

NP_006556.1 (Human)

Additional CTCF Products

Product Documents for CTCF Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CTCF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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